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Postnatal day 2 mouse brain was electroporated with CMV-GFP plasmid into the anterior lateral ventricle (LV), cut into 250 mm thick slices and cultured in vitro for 3 days on polycarbonate membrane filters in serum-free defined media. GFP-positive cells (green) are seen in the SVZ and a few are migrating toward the olfactory bulb. The slice was counterstained with propidium iodide (red). The inset is an example of a slice prepared from another animal and shows the GFP-labeled cells migrating in the rostral migratory stream. *, anterior subventricular zone; OB, olfactory bulb.