Our laboratory is using zebrafish as a model system to study the regulation of persistent olfactory bulb neurogenesis.  We are collaborating with Daniel Goldman, Ph.D., who developed a transgenic zebrafish in which the a1-tubulin promoter drives expression of a green fluorescent protein (GFP) reporter. This promoter restricts GFP expression to newly generated or regenerating neurons.  Horizontal sections through 3-5 week old transgenic zebrafish immunostained for bromodeoxyuridine (BrdU) after pulse BrdU labeling shows newly generated,BrdU-labeled cells (left panel) in a pattern that overlaps with immature, GFP-expressing neurons (right panel). The newborn neurons are present in the midline telencephalon (Tel), olfactory bulbs (OB) and olfactory epithelium (OE).  We are currently using a mutagenesis screen of these transgenic zebrafish to identify genes that regulate forebrain neurogenesis.