|YANG RESEARCH GROUP - Red Blood Cells as Carriers|
|Red Blood Cells As Drug
L-Asparaginase Delivery Using Erythrocytes as Carrier
L-Asparaginase (ASNase) is a drug used in treatment of acute lymphoblastic leukemia (ALL). Although it has been in use for over 30 years, due to its bacterial source, immunogenicity has been a major problem. Short half-life of the enzyme also makes frequent injection necessary, exacerbating the problem. In order to address these issues, we propose encapsulating ASNase into red blood cells (RBCs). Previously, drug-induced endocytosis, electroporation, and various form of hypo-osmotic methods have been used for drug encapsulation into RBCs. However, these methods result in alterations to RBC morphology and cell membrane characteristics, which in turn increases chance for RBC removal from the system. By conjugating ASNase to protein transduction domain peptide (PTD), we can load the enzyme into RBCs with minimal changes to these parameters and thereby increase circulation time in vivo and at the same time reduce immunogenic
effect from the drug.
Long-Circulating Cocaine Esterase (CocE)