Methods: Biofilms formed by yeasts after 48 h in the bottom of 96-well microtiter plates were treated with the photosensitizer (ZnPc) and a GaAlAs laser (26.3 J.cm-2). The biofilm cells were scraped off the well wall, homogenized and seeded onto Sabouraud dextrose agar plates that were then incubated at 37ºC for 48 h. Efficient PDI of biofilms was verified by counting colony-forming units (CFU/mL), and the data were submitted to analysis of variance and the Tukey test (P < 0.05).
Results: All biofilms studied were susceptible to PDI with statistically significant differences. The strains of Candida genus were more resistant to PDI than emerging pathogens T. mucoides and K. ohmeri. A mean reduction of 0.45 log was achieved for Candida spp. Biofilms, and a reduction of 0.85 and 0.84 was achieved for biofilms formed by T. mucoides and K. ohmeri, respectively.
Conclusions: Therefore, PDI by treatment with nanostructured formulations cationic zinc 2,9,16,23- tetrakis (phenylthio)- 29H, 31H- phthalocyanine (ZnPc) and a laser was effective on biofilms formed by strains of C. albicans and non-Candida albicans as well the emerging pathogens T. mucoides and K. ohmeri.
Keywords: Biofilm, Fungi, Lasers and Microbiology