"Methods: " In this lab trial study, PDL cells were obtained from 124 healthy anterior and posterior sheep teeth and cultured in αMEM, DMEM, HBSS, and mint extract for periods of 2, 6, 24, 48, 72, or 96 hours (24 groups). For each solution, positive control group was considered those evaluated PDL cells without incubation in any storage condition. For each group, there was a negative control considered cells growing in dry plate with no medium. After exposure of PDL cells to scheduled solution for scheduled incubation time, centrifuge was performed for 10 minutes. Then collagenase (3mgr/ml) and Dispase (4mgr/ml) were added to cell precipitates which were incubated at 37° C for 60 minutes. After washing cellular suspension in PBS, vitality of the cells was assessed by Trypan blue exclusion. The data was analyzed statistically using 2-way ANOVA test.
"Results: " Statistically significant differences in efficacy of different medias were obtained at least between two conditions (P=0.0001). PDL cells cultured in αMEM and mint extract showed 90% and 52.22% vitality representing the best and the worst storage media, respectively.
"Conclusions: " αMEM can be a suitable transport medium up to 96 hours to preserve the vitality of the PDL cells of avulsed teeth. There is a reverse correlation between the viability of PDL cells and incubation time.
Keywords: Animal, Cell culture, Periodontium-gingiva, Teeth and storage media
See more of: Periodontal Research - Therapy