E2F3

General Information

Full gene name:E2F transcription factor 3 Entrez Gene ID:1871 Location:6p22 Synonyms:E2F-3 Type:protein-coding

User SNPs

SNPs given by the user that are near or inside this gene:

SNP	Distance (bp)	Direction
rs7754840	167305	downstream

NCBI Summary

The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F1 and E2F2, have an additional cyclin binding domain. This protein binds specifically to retinoblastoma protein pRB in a cell-cycle dependent manner. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2011]

OMIM

OMIM ID:`OMIM ID 600427 `_

NCBI Phenotypes

No phenotypes found linked to this gene.

Gene Ontology

• mitotic cell cycle
• transcription initiation from RNA polymerase II promoter
• G1 phase of mitotic cell cycle
• nucleoplasm
• protein binding
• G2 phase of mitotic cell cycle
• sequence-specific DNA binding transcription factor activity
• positive regulation of transcription, DNA-dependent
• regulation of transcription, DNA-dependent
• transcription factor complex
• positive regulation of cell proliferation
• core promoter binding

KEGG Pathways

• Prostate cancer
• Melanoma
• Glioma
• Bladder cancer
• Small cell lung cancer
• Non-small cell lung cancer
• Pathways in cancer
• HTLV-I infection
• Pancreatic cancer
• Chronic myeloid leukemia
• Cell cycle

GeneRIFs

• Affinity Capture-MS [PMID 16147992]
• E2F3 (E2F-3) interacts with the BIRC5 (survivin) promoter. [PMID 15271987]
• just as E2F3, HELLS is overexpressed in human tumours including prostate cancer, indicating that either factor may contribute to the malignant progression of tumours [PMID 22157815]
• Affinity Capture-Western [PMID 22157815]
• Affinity Capture-Western [PMID 22157815]
• Affinity Capture-Western [PMID 22157815]
• Affinity Capture-MS; Affinity Capture-Western; Reconstituted Complex [PMID 22157815]
• Affinity Capture-Western [PMID 22157815]
• E2F3 interacts with the B-myb promoter region. [PMID 10766737]
• E2F3 interacts with the Cdc25A promoter. [PMID 10766737]
• E2F3 interacts with the Cdc6 promoter. [PMID 10766737]
• E2F3 interacts with the Cdc2 promoter. [PMID 10766737]
• Data show that E2F is likely a central coordinator of multiple responses that culminate in regulation of EBP1 gene expression, and which may vary depending on cell type and context. [PMID 21085677]
• E2F3 mRNA and protein are overexpressed in retinoblastoma. [PMID 16180235]
• E2F3 interacts with EAPP. [PMID 15716352]
• A significant elevation in expression of E2F3 in oral squamous cell carcinoma cells was seen in carbon and neon-irradiated cells. [PMID 18514338]
• E2F3 interacts with the MT1G promoter. [PMID 15735762]
• determination of interaction with RYBP and YY1 [PMID 12411495]
• Affinity Capture-Western; Two-hybrid [PMID 12411495]
• Two-hybrid [PMID 12411495]
• Two-hybrid [PMID 12411495]
• E2F3 interacts with RYBP. [PMID 12411495]
• E2F3 interacts with a molecule expressed by Gene FHL2. The precise molecular variant of FHL2 involved in this interaction is not specified. [PMID 12411495]
• the hypoxia component of ischemia may limit wound re-epithelialization by stabilizing HIF-1alpha, which induces miR-210 expression, resulting in the down-regulation of the cell-cycle regulatory protein E2F3 [PMID 20308562]
• E2F3 gain is associated with the late stage of retinoblastoma. [PMID 18785023]
• We conclude that E2F3 is frequently amplified and overexpressed in invasively growing bladder cancer (stage pT1-4). E2F3 expression appears to provide a growth advantage to tumor cells by activating cell proliferation in a subset of bladder tumors. [PMID 15122326]
• E2F1, E2F2, and E2F3 directly bind the promoter of the mir-17-92 cluster, activating its transcription. miR-20a from the mir-17-92 cluster in turn modulates the translation of E2F2 and E2F3, suggesting an autoregulatory feedback loop. [PMID 17135249]
• Observational study of gene-disease association and gene-environment interaction. (HuGE Navigator) [PMID 21048031]
• E2F3 interacts with the JMY promoter. [PMID 15706352]
• E2F3 interacts with the PPP1R13B (ASPP1) promoter. [PMID 15706352]
• E2F3 interacts with the TP53BP2 (ASPP2) promoter. [PMID 15706352]
• E2F3 interacts with the TP53INP1 promoter. [PMID 15706352]
• E2F3a induces apoptosis in HepG2 cells and plays important roles in regulating transcription [PMID 20803551]
• E2F3 levels have a critical role in modifying cellular proliferation rate in human bladder and prostate cancer. [PMID 16909110]
• Inactivation of the retinoblastoma pathway occurs in bladder tumors with 6p22 amplification. [PMID 18037967]
• Observational study of gene-disease association. (HuGE Navigator) [PMID 19738611]
• requirement of E2F3 for BCR/ABL leukemogenesis [PMID 17925491]
• Affinity Capture-Western [PMID 16951254]
• Genes involved in the E2F3 pathway are associated with chemotherapy sensitivity among ER- tumors. The mutant p53 signature and expression of ER-related genes were associated with lower sensitivity to chemotherapy in ER+ breast tumors only. [PMID 18445275]
• The Gab2 promoter region interacts with E2F3. [PMID 15574337]
• Expression of E2F3 was lowest in early-stage tumors and highest in metastatic tissue. Expression profiling of miRNAs in WT showed that expression of each measured member of the Oncomir-1 family was highest in WT relative to other kidney tumor subtypes [PMID 18519660]
• Reconstituted Complex [PMID 8657141]
• E2F3 interacts with the MSH2 promoter. [PMID 11799067]
• E2F3 interacts with the UXT chromatin. [PMID 11799066]
• Study demonstrates the strength of E2F3 as a potential marker for discriminating benign and malignant disease, addressing the current limitations of serum PSA measurements. [PMID 17701752]
• miR-203 induced cellular senescence by targeting E2F3 in human melanoma Mewo and A2058 cells [PMID 22354972]
• E2F-3 interacts with the E2F site. This interaction was modelled on a demonstrated interaction between E2F-3 and a synthetic dsDNA construct. [PMID 8246996]
• Affinity Capture-Western; Co-localization [PMID 15014447]
• E2F3 is overexpressed in 55-70% of squamous cell carcinomas and 79% of adenocarcinomas of the lung. In addition very high level expression of nuclear E2F3 is found in almost all small cell lung cancers analysed. [PMID 16938365]
• E2F3 contributes to ataxia telangiectasia mutated (ATM) kinase-dependent p53 phosphorylation and apoptosis in cells expressing E1A. [PMID 18235226]
• E2F3 modulates Aurora-A mRNA expression during the cell cycle. [PMID 18776222]
• Data report that ANCCA directly interacts with E2F1 to E2F3 in its complex with MLL and that its N terminus interacts with both the N and C termini of E2F1. [PMID 20855524]
• Affinity Capture-Western; Reconstituted Complex [PMID 20855524]
• Affinity Capture-Western; Two-hybrid [PMID 17612494]
• miR-34a targeting the Sirt1 and E2F3 genes could negatively regulate the resistance to 5-FU in human colorectal cancer DLD-1 cells. [PMID 21067862]
• these data reveal the novel ability of E2f3 to function as a master regulator of the DNA damage response. [PMID 19917728]
• results suggest that DEK and E2F3 are potential targets of 6p gains in retinoblastoma [PMID 16007192]
• In bladder cancer, the E2F3 gene is associated with overexpression of its encoded mRNA transcripts and high levels of E2F3 expression. [PMID 14716298]
• report confirms significant mRNA overexpression of KIF14 and E2F3 together in a large cohort of retinoblastoma tumors [PMID 19190782]
• miR-128 can inhibit proliferation of glioma cells through one of its targets, E2F3a. [PMID 18810376]
• human Pumilio homologs Pum 1 and Pum 2 repress the translation of E2F3 by binding to the E2F3 3’ untranslated region (UTR) and also enhance the activity of multiple E2F3 targeting microRNAs (miRNAs) [PMID 22345517]
• Data suggest that the physical interaction of TFE3 and E2F3 facilitates transcriptional activation of the p68 gene and provides strong evidence for the specificity of E2F function. [PMID 12748276]
• Two-hybrid [PMID 12748276]
• Two-hybrid [PMID 12748276]
• Affinity Capture-Western; Reconstituted Complex; Two-hybrid [PMID 12748276]
• Two-hybrid [PMID 12748276]
• Two-hybrid [PMID 12748276]
• Two-hybrid [PMID 12748276]
• Two-hybrid [PMID 12748276]
• Two-hybrid [PMID 12748276]

PubMed Articles

Recent articles:

• Noguchi S et al. “Anti-oncogenic microRNA-203 induces senescence by targeting E2F3 protein in human melanoma cells.” J Biol Chem. 2012 Apr 6;287(15):11769-77. PMID 22354972
• Miles WO et al. “Pumilio facilitates miRNA regulation of the E2F3 oncogene.” Genes Dev. 2012 Feb 15;26(4):356-68. PMID 22345517
• von Eyss B et al. “The SNF2-like helicase HELLS mediates E2F3-dependent transcription and cellular transformation.” EMBO J. 2011 Dec 13;31(4):972-85. PMID 22157815
• Casabonne D et al. “Single nucleotide polymorphisms of matrix metalloproteinase 9 (MMP9) and tumor protein 73 (TP73) interact with Epstein-Barr virus in chronic lymphocytic leukemia: results from the European case-control study EpiLymph.” Haematologica. 2011 Feb;96(2):323-7. PMID 21048031
• Akao Y et al. “Dysregulation of microRNA-34a expression causes drug-resistance to 5-FU in human colon cancer DLD-1 cells.” Cancer Lett. 2011 Jan 28;300(2):197-204. PMID 21067862
• Li W et al. “Characterization of E2F3a function in HepG2 liver cancer cells.” J Cell Biochem. 2010 Dec 1;111(5):1244-51. PMID 20803551
• Judah D et al. “EBP1 is a novel E2F target gene regulated by transforming growth factor-β.” PLoS One. 2010 Nov 10;5(11):e13941. PMID 21085677
• Revenko AS et al. “Chromatin loading of E2F-MLL complex by cancer-associated coregulator ANCCA via reading a specific histone mark.” Mol Cell Biol. 2010 Nov;30(22):5260-72. PMID 20855524
• Biswas S et al. “Hypoxia inducible microRNA 210 attenuates keratinocyte proliferation and impairs closure in a murine model of ischemic wounds.” Proc Natl Acad Sci U S A. 2010 Apr 13;107(15):6976-81. PMID 20308562
• Martinez LA et al. “E2F3 is a mediator of DNA damage-induced apoptosis.” Mol Cell Biol. 2010 Jan;30(2):524-36. PMID 19917728

Top Pubmed articles linked to gene E2F3 matching any search term:

• Ignatiadis M et al. “Gene modules and response to neoadjuvant chemotherapy in breast cancer subtypes: a pooled analysis.” J Clin Oncol. 2012 Jun 1;30(16):1996-2004. PMID 22508827
• Real S et al. “E2F1 regulates cellular growth by mTORC1 signaling.” PLoS One. 2011 Jan 24;6(1):e16163. PMID 21283628
• von Willebrand M et al. “The tyrphostin AG1024 accelerates the degradation of phosphorylated forms of retinoblastoma protein (pRb) and restores pRb tumor suppressive function in melanoma cells.” Cancer Res. 2003 Mar 15;63(6):1420-9. PMID 12649208