Method: Probase ColdCured(PB) (IvoclarVivadent), Lucitone199-Compression(LC) (Dentsply), Lucitone199-Success(LS) (Dentsply), Ivobase-Hybrid (IB) (IvoclarVivadent) and Ivocap Injection(IC) (IvoclarVivadent) were used. Eight specimens 10x10x3 mm were fabricated from each material. The specimens were sequentially finished, polished with pumice slurry and a felt cone, ultrasonically cleaned, and stored in water for 24h. Half of the specimens of each material were immersed in human saliva for 30 min. All the specimens were inoculated with a C.albicans culture and incubated at 37 °C for 30 min. The number of C.albicans cells attached to the surface was counted under light microscope and averaged for 10 fields of view per sample. ANOVA on Ranks followed by Tukey test was used respectively for the saliva treated and not saliva treated groups to identify differences. Mann-Whitney test was used to determine the presence of significant differences between groups of the same material treated and not treated with saliva. Significance level was set at 0.05.
Result: Nominally, all the groups showed higher counts of C.albicans on the samples treated with saliva. However, a significant difference was detected only for LS (P=0.029). For samples not treated with saliva, median count values per field of view ranged from 7 (IB) to 65 (PB). Significant differences among the groups were detected between IB and LC (P=0.027). Median count values per field of view ranged from 10 (IB) to 91 (LS) after the samples had been immersed in saliva. Differences among materials were also found to be significant in this case (P=0.006). IB was found to adhere significantly less C.albicans than LS and LC.
Conclusion: The number of C.albicans that adheres to the denture surfaces can change dramatically depending on each specific material. Study partially funded by Ivoclar-Vivadent.
Keywords: Acrylics, Adherence and colonization, Dental materials and Prosthodontics