Results: The average size of monkey Aa genomes was 2.4 Mbp (GC=44.4%), and that of human Aa was 2.2 Mbp (GC=44.2%). Annotation using the tools RAST and GLIMMER v3.02 revealed on average 2416 genes for monkey Aa and 2123 genes for human Aa. Except for one rhesus isolate, all 11 monkey Aa isolates and two human isolates possessed genes coding for major virulence factors of Aa, leukotoxin, CDT, tad operon, and adhesins ApiA and Aae. All Aa isolates had a non-JP2 leukotoxin promoter. Each of the above sequences when aligned with corresponding sequences from human Aa isolates exhibited 90-95% identity. Furthermore, genomic islands coding for O-antigen and Lipooligosaccharide, in addition to 3 uncharacterized genomic islands, were found in all isolates. Extensive analyses of genome sequences of the Aa isolates is under progress. Conclusion: Comparative genomics might help elucidate evolutionary significance of genome level differences among Aa isolates, and perhaps also to translate those differences into Aa’s role in periodontitis in humans and in monkeys.
Keywords: Genomics, Oral biology and Periodontal disease
See more of: Periodontal Research - Pathogenesis