Method: Osteoblast (MC3T3-E1) and osteoclast (RAW264.7) cell lines were used. MnTE-2-PyP was added (10 μg/ml) into the cell cultures 1 hr before irradiation. MC3T3-E1 and RAW cells were then exposed to gamma-ray irradiation at total doses of 0, 2, 4, 6, 8 and 10 Gy. Cell viability and caspase 3/7 activation (apoptosis) were tested by ApoLive-Glo™ Multiplex Assay (Promega). The GSH-Glo™ Glutathione Assay (Promega) was used for the quantification of glutathione (GSH) as an indicator of toxicological responses or oxidative stress. Cell assays were performed 48h post-irradiation to test the effect of MnTE-2-PyP with and without radiation on cells.
Result: For cell viability, MnTE-2-PyP significantly decreased radiation damage on MC3T3-E1 and RAW cells at all doses (P<0.05). On the contrary, caspase3/7 apoptosis levels were significantly decreased in irradiated cells treated with MnTE-2-PyP than their counterparts without MnTE-2-PyP (P<0.05). In addition, the oxidative stress indicated by GSH level were significantly lowered in MnTE-2-PyP -treated cells at all the radiation doses (P<0.05).
Conclusion: MnTE-2-PyP might protect osteoblast and osteoclast during radiotherapy via decreasing the oxidative stress and apoptosis in vital cell organelles.
Keywords: Apoptosis, Osteoblasts/osteoclasts and irradiation
See more of: Craniofacial Biology