Objective: The aim of this study was to investigate whether COMP is expressed by PDL cells and how its expression might be regulated by factors that control TGF-β activity. Based on gene array data, we hypothesize that TSP-1 increases TGF-β activity and COMP expression in PDL cells.
Method: Human PDL cells were treated with TSP-1 (10-100nM) and TGF-β1 (200pM) for 2 to 72 hours. Quantitative RT-PCR analyses were performed using COMP and S9 (housekeeping gene control) primers.
Result: Both TSP-1 and TGF-β1 treatment of PDL cells increased COMP mRNA as measured by RTq-PCR. Further studies are being conducted to determine whether TSP-1 stimulation of COMP occurs through TSP-1-dependent TGF-β activation or by direct stimulation.
Conclusion: This is the first report of COMP regulation by TSP-1 and of TGF-β1 regulation of COMP mRNA in PDL cells. These data suggest a novel pathway for COMP regulation and suggest that COMP might play a role in periodontal ligament function, regeneration and repair.
This work is supported by NIDCR F30 DE018259.
Keywords: Cell culture, Extracellular matrix molecules and Fibroblasts
See more of: Periodontal Research - Pathogenesis