Method: 1) Dmp1 null mice and Hyp mice were compared at different stages, 3-week, 6-week, and 3-month by histology and Micro-CT; 2) mechanical properties of Dmp1 null and Hyp bone were studied by 3-point bending test; 3) soft diet was used to decrease the occlusal loading in an attempt to rescue the periodontal bone loss in Dmp1 null mice.
Result: Although Dmp1 null mice and Hyp mice share some similarities in their tooth and long bone phenotypes, much less periodontal bone loss was observed in Hyp mice. However, 3-point bending test showed similar Young’s modulus in Dmp1 null bone and Hyp bone, suggesting that occlusal loading would lead to increased bone strain in both mice, compared with the wildtype control. Lastly, soft diet from 3-week to 6-week significantly decreased the periodontal bone loss in Dmp1 null mice, but didn’t fully rescue the phenotype.
Conclusion: Both local and systemic effects of deleting DMP1 may be responsible for the periodontal bone loss in Dmp1 null mice.
Keywords: Bone and Loading