Method: A total of 80, 5-12 year-old children were recruited from the out-patient clinic at School of Dentistry, University of Detroit Mercy. Whole saliva stimulated by paraffin-chewing was collected and children were investigated for decayed, missing, and filled surfaces (DMFS) according to WHO criteria. Oral hygiene was determined according to the simplified Oral Hygiene Index (OHI-S) of Greene and Vermillion. The Dentocult® SM and Dentocult® LB Strips were used to estimate S. mutans and Lactobacillus (LB) count in stimulated saliva. The level of salivary IgA antibody against SBR and S. mutans were determined by enzyme-linked immunosorbent assay (ELISA). Data subjected to statistical analysis.
Result: The results of our study showed that children with low caries prevalence had a significantly higher amount of salivary IgA antibody against SBR / S. mutans compared to those with high caries prevalence. Furthermore, a significant correlation between salivary Lactobacillus counts, salivary flow rate and caries prevalence were also observed.
Conclusion: The results of our study suggest that salivary IgA antibodies to SBR and S. mutans may play an important role in developing a protective immunity to dental caries in School-aged children. Our results also demonstrated that salivary S. mutans and Lactobacillus are strongly correlated with caries prevalence and should be considered as risk factors for dental caries.
Keywords: Caries, IgA, Immune response, Oral hygiene and Saliva
See more of: Cariology Research - Detection, Risk Assessment and Others