1417 Evaluation of Fluoride Rinses Employing an in vitro Remineralization/Demineralization Model

Saturday, March 24, 2012: 9:45 a.m. - 11 a.m.
Presentation Type: Poster Session
E.E. ENGELMAN1, F. PASCAL1, S. SATTAR1, B. SCHEMEHORN2, and R.J. GAMBOGI1, 1Johnson & Johnson Consumer Companies, Inc, Skillman, NJ, 2Dental Product Testing, Therametric Technologies, Inc, Noblesville, IN
Objective: Determine the impact of phosphate and sodium lauryl sulfate (SLS) on enamel fluoride uptake; lesion remineralization; and prevention of demineralization on acid challenge.

Method: Artificial lesions were formed in human enamel specimens by immersion in lactic acid/Carbopol, 50% saturated with hydroxyapatite(pH 5.0).  A remineralizing solution was used as the medium in all treatment groups.  After initial surface microhardness (SMH), specimens were treated in the following sequence for 15-days: (1 min treatment, remineralization, 1 min treatment, remineralization, acid challenge, remineralization, 1 min treatment, remineralization, 1 min treatment followed by remineralization overnight).  Specimens were analyzed for SMH (5, 10, and 15-days) and lesion fluoride content (microdrilling method; 15-days).  Following the fluoride analyses, specimens were subjected to a simulated plaque acid challenge (SPAC).  The control rinse (CR) consisted of 21.6%v/v ethanol, 0.0221%w/v NaF ( pH=3.5).  The other treatment samples were CR+0.1M Phosphate and CR+0.1M Phosphate+0.1%w/w SLS. 

Result:

Treatment Group

Δ Surface Microhardness (Mean+/-SD)

Fluoride Content mgF/cm3

5-Day

10-Day

15-Day

SPAC

H2O

2.8±1.4

2.3±1.9

0.5±1.5

-7.5±1.4

217±7

CR

10.4±1.5a

12.2±1.6a

20.3±1.9a

6.1±1.5a

2,579±101a

CR+Phosphate

15.9±1.8a

20.1±1.9b

25.4±2.1a

13.6±2.2b

2,920±134b

CR+Phosphate+SLS

11.9±1.5a

15.9±1.5a,b

22.7±1.6a

9.9±1.6a,b

3,132±127b

All three rinses remineralized lesions significantly better than H2O at all time points.  For each column, values with identical letters do not differ significantly (p>0.05) as determined by SNK analysis.  Both phosphate-containing rinses were significantly more effective in promoting fluoride uptake.  CR+Phosphate was more effective remineralizing lesions than the control at 10-days and in the SPAC.  No significant differences were found between the CR+Phosphate and CR+Phosphate+SLS mouthrinses. 

Conclusion: The addition of sodium lauryl sulfate did not impede fluoride efficacy in these low pH, hydroalcoholic mouthrinses.  When phosphate was present (with and without SLS), rinses displayed a superior benefit in fluoride uptake compared to the positive control.


Keywords: Demineralization, Fluoride, Mouthrinses and Remineralization