345 Effects of Abrasive Blasting on the Response of MC3T3-E1 Cells

Thursday, March 22, 2012: 2 p.m. - 3:15 p.m.
Presentation Type: Poster Session
J. PANTHACHAI1, M. ARKSORNNUKIT1, and P. PAVASANT2, 1Department of Prosthodotics, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand, 2Department of Anatomy, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand

Objective : The aim of this study was to compare the biological effects of titanium surface prepared from two different abrasive materials, Al2O3 and glass beads (SiO2). 

Methods : Commercially pure grade 2 titanium discs were sandblasted with either 50 µm of Al2O3 (Dental vision) or SiO2 (Accord). A polished titanium discs were prepared for control group. The surface roughness and topography after sandblasting were evaluated by profilometry (Talyscan 150, UK, n=10) and scanning electron microscopy, while chemical composition was investigated with energy dispersive X-ray spectroscopy (EDS).  The adhesion and proliferation of MC3T3-E1 on different Ti surface was determined by MTT assay. The expression of osteopontin (OPN) and osteocalcin (OC) at day-7 were examined by reverse transcription polymerase chain reaction.  The ability of Ti surface on supporting fibrin formation was evaluated. Data were expressed as mean± standard deviation. Statistical analysis was carried out by one-way ANOVA, follow by Scheffe test at p<0.05.

Results : Showed the comparable roughness values for the surface blasted with Al2O3 (Ra=0.2349 µm) and SiO2 (Ra=0.2145 µm).  However, blasting with Al2O3 yielded more wettablity surface. In addition, Al2O3 blasted surface support higher amount of fibrin formation after 5 min.  Cells seeded on Al2O3 blasted surface also showed significant faster rate of adhesion at 30 min and higher rate of proliferation at day-2 (p<0.05). In this study, the expression of OPN from both blasted groups was comparable. Moreover, OC which is considered as a marker of osteoblast differentiation, indicated that Al2O3 blasted surfaces possess higher expression than SiO2 blasted surfaces.

Conclusion : The Al2O3 blasted surface could support the osteoblast adhesion and differentiation better than SiO2. These results revealed that different sandblast materials could give different surface property leading to different cellular responses and the information should be more concern by the clinician.



Keywords: Blasting, Cell culture, Gene expression and Implants