Method: DNA was isolated from paraffin-embedded labial mucosa and lip samples from the University of Louisville Oral Pathology Laboratory (LOPL). Examination of the clinical and histological records of the two patients suggested focal epithelial hyperplasia (FEH). Theuniversal HPV primers My09/My11were used for PCR amplification and the resulting products were sequenced. Separately, DNA was isolated from one frozen-tissue sample from a canine anogenital biopsy. Subsequent amplification was accomplished by the multiply primed rolling-circle technique to obtain the viral plasmid. The circular DNA was linearized and cloned into a pUC19 vector. All genomic and phylogenetic comparison was completed using theNCBI BLAST database and DNAStar software.
Result: Samples from the LOPL harbored HPV32, supporting the diagnosis of FEH. Whole viral genome isolation from the canine anogenital biopsy indicated the presence of canine oral papillomavirus (COPV).
Conclusion: The universal HPV primers My09/My11 were used to confirm a diagnosis of FEH in two patients. Furthermore, using whole genome purification and analysis we indicate a novel anogenital tropism for COPV. These two approaches for PV identification emphasize the relative simplicity of PV detection and highlight the power of molecular biology techniques to inform the researcher and clinician.
Keywords: Infection, Methodology, Molecular biology, Papillomavirus and Pathology