Method: The 17IIA11 preodontoblastic cell line, which expresses odontoblast markers and is capable of producing mineralizing matrix (when cultured in differentiation conditions) was used to generate a Trps1-deficient line by shRNA-mediated knockdown. Effects of Trps1 deficiency on mineralization were evaluated by alkaline phosphatase and alizarin red staining. Changes in gene expression pattern were analyzed by an Affimetrix Mouse Exon 1.0 ST Array and confirmed by qRT-PCR. In addition, expression of selected genes was analyzed in teeth of Trps1 mutant mice by IHC.
Result: Trps1 deficiency in the 17IIA11 preodontoblastic cell line results in loss of their mineralization potential. Several genes required for dentin formation and the mineralization process were significantly downregulated in Trps1 deficient cells.
Conclusion: Our studies demonstrate that Trps1 is required for expression of genes critical for mineralization and may be important for odontoblast terminal differentiation.
Keywords: Dentin, Gene expression, Mineralization and Odontoblasts
See more of: Mineralized Tissue