Objectives: This study is to evaluate in vitro and in vivo bone regenerative capability of MSCs with intracellular release of E2.
Methods: After rat bone marrow MSCs took up E2-loaded PLGA microparticles, their proliferation and in vitro osteogenic differentiation capabilities were measured using DNA quantification and real time PCR. In vivo bone regeneration of these MSCs was investigated using a rat calvarial bone defect model.
Results: Rat MSCs with intracellular release of E2 delivered by PLGA microparticles exhibited significantly higher proliferation rate compared to the controls. mRNA expression of osteocalcin, an osteogenic differentiation marker, of treated MSCs was significantly up-regulated compared to control groups after they were exposed to osteogenic differentiation medium in vitro. Bone regeneration at calvarial defects treated by MSCs with uptake of E2-loaded PLGA particles are faster than controls.
Conclusions: Intracellular release of E2 delivered by PLGA microparticles effectively enhanced proliferation and bone regenerative capabilities of MSCs. This approach can be potentially used to improve the capabilities of MSCs for MSC-based bone tissue engineering.
Keywords: Bioengineering, Bone, Bone repair and Tissue engineering