Methods: JET acrylic discs pre-conditioned with saliva were incubated with C. albicans cell suspension in the presence and absence of Hst-5 under various experimental conditions. Following rinsing, adherent cells were recovered by sonication, diluted and plated on fungal media for quantification of colony forming units (CFUs) in order to assess fungal burden. The rat denture model was successfully tested for suitability as a model to study the development and progression of DS.
Results: Hst-5 treatment of discs resulted in significant decrease (p<0.05) in level of C. albicans adherence and subsequent colonization. Protective effect of Hst-5 was proportional to its concentration and inversely proportional to C. albicans cell density.
Conclusions: These in vitro findings demonstrate the efficaciousness of Hst-5 in preventing colonization of denture material attributing a therapeutic potential for the prevention of oral prosthesis. Currently, studies to demonstrate this potential in vivo are underway using the rat model. This is the first study exploring the application of Hst-5 using a combination of in vitro and animal studies.
Keywords: Adherence and colonization, Antimicrobial agents/inhibitors, Biofilm, Fungi and Saliva