2.    Dermatologic manifestations of systemic disease (e.g. neoplasia, inflammatory bowel disease, meningococcemia, systemic lupus erythematosus)

From various sources:

Café au lait spots

Neurofibromatosis

hyperpigmentation

Addison’s disease

Koplik spots

measles

Erythema chronicum migrans

Lyme disease

myxedema

Hypothyroidism

Palpable purpura on legs/buttocks

Henoch-Schonlein purpura

Port wine stain

hemangioma

Rash on palms and soles

Secondary syphilis

Rocky Mountain spotted fever

meningococcemia

Malar rash, discoid rash

Lupus (SLE)

“slapped cheeks”

Fifth disease (erythema infectiosum)

Osler’s nodes, Janeway lesions

Bacterial endocarditis

Erythema marginatum (distinctive migratory and transient pink rash with pale centers)

Rheumatic fever

Raynaud’s phenomenon

Buerger’s disease, CREST (scleroderma)

Caput medusae

Portal HTN

Erythema nodosum, pyoderma gangrenosum

Ulcerative colitis

 

 

 

 

 

 

 

 

 

From lecture (12/21/00):

Neoplasia

Cutaneous metastasis

-75% are first sign of extranodal spread

-common primaries: breast, lung, GI and skin

-firm papules/nodules, often bound down; ulcers

Leukemia cutis

-localized or disseminated skin infiltration by leukemic cells

-diffuse, small pinkish, non-tender papules

-herald presence of leukemia cells in peripheral circulation

Paraneoplastic pemphigus

-autoimmune blistering condition affecting skin and mucosa

-indirect immunofluorescence: binding to simple, columnar and transitional epithelia (chicken wire staining)

-vermillion border involvement is characteristic

-associated w/ CLL, large cell lymphoma, non-Hodgkin’s, Waldenstrom’s macroglobulinemia

Bullous neutrophilic dermatosis

-rapidly expanding painful, ulcerative nodule

-looks like bad infection, but aseptic w/ hemorrhagic border

-lesions form after trauma (Pathergic response)

-associated w/ lymphoreticular system malignancies

-treat w/ steroids or immunosuppressive agents

GI

Peutz Jehgers

-autosomal dominant polyposis w/ hyperpigmented macules

-hyperpigmentation of lips, buccal mucosa, palms/soles, periorbital

-hamatomatous polyps in small intestine mainly

-Sx: recurrent attacks of severe abdominal pain (intussusception, obstruction, rectal prolapse, GI bleeding)

-rarely associated w/ gondal tumors, breast carcinoma

Acrodermatitis enteropathica

-disorder of zinc absorption – genetic or acquired

-triad: acral dermatitis (hands/feet, face, anogenital), alopecia, diarrhea

-dry, scaly eczematous patches and plaques

Glucagonoma syndrome

-excessive production of glucagons by a-cell tumor of pancreas

-lesions w/ central clearing and blistering/crusting edges

-periorificial and intertriginous dermatitis/erythema

-glossitis and angular chelitis also seen

Gardner’s syndrome

-autosomal dominant intestinal (colonic) polyposis

-high rate of malignant transformation

-multiple epidermoid cysts, esp of face, scalp and trunk

-osteomatosis of maxilla, mandible and cranial bones

-fibrous tumors of skin and subcutaneous tissue

Muir-Torre syndrome

-sebaceous tumors most often on face and trunk

-assoc. w/ visceral neoplasms, esp colon, also larynx & endometrium

Endocrine & Metabolic

Necrobiosis lipoidica diabeticorum

-sharply circumscribed atrophic plaques w/ yellowish-brown color

-classical location: anterior and lateral surfaces of lower legs

-2/3 have overt diabetes mellitus

Pretibial myxedema

-bilateral indurated plaques and nodules (orange peel-like)

-pretibial region of lower extremities

-occurs w/ Grave’s disease, recovery from “thyroid storm”

Acanthosis Nigrican

-diffuse velvety thickening and hyperpigmentation

-typically on axilla, other body folds, knuckles

-associated w/ endocrine disorders (IDDM, acromegaly, Cushing’s, Addison’s), drugs, paraneoplastic (adenocarcinoma)

Cowden’s disease

-multiple wart-like lesions around mouth, nose, ears

-multiple popular lesions (“cobblestones”) on mucosal membranes

-increased risk of breast and thyroid cancers

 


 

4.     Renal failure: acute versus chronic, features of uremia

 

Acute

Chronic

-quicker onset

-usually reversible

-progressive

-usually irreversible w/ scarring

-leads to end stage renal disease

 

Causes of renal failure

prerenal

renal

postrenal

-hypovolemia

-cardiac failure

-hepatorenal syndrome

-NSAIDs

-ACE inhibitors

-vascular disorders

-glomerulonephritis

-interstitial nephritis

-tubular necrosis

-extrarenal obstruction

-bladder rupture

 

Uremia – signs and symptoms of toxin accumulation when kidney fails. Clinical characteristics:

  1. Azotemia – increased serum creatinine and/or urea
  2. acidosis – from accumulation of sulfates, phosphates, and organic acids
  3. hyperkalemia
  4. abnormal control of fluid volume – early: inability to concentrate urine; late: inability to dilute; sodium and water retention -> CHF
  5. hypocalcemia – failure to make active form of vitamin D -> renal osteodystrophy
  6. anemia – decreased secretion of erythropoietin
  7. hypertension – hyperproduction of rennin

 

BRS Pathology, lecture (12/6/00)


 

5.     Acid-base disturbances, including renal tubular acidosis

 

 

pH

PCO2

[HCO3-]

causes

Compensatory response

Respiratory acidosis

­

< 7.4

­

> 40 mmHg

­

COPD, airway obstruction                                                                          

Renal [HCO3-] reabsorption

Respiratory alkalosis

­

> 7.4

¯

< 40 mmHg

¯

High altitude, hyperventilation

Renal [HCO3-] secretion

Metabolic acidosis

(check anion gap)

¯

< 7.4

¯

< 40 mmHg*

¯

DKA, diarhhea, lactic acidosis, salicylate OD, acetazolamide OD, renal failure

hyperventilation

Metabolic alkalosis

¯

> 7.4

­

> 40 mmHg*

­

vomiting

hypoventilation

* with compensation

 

 

 

Metabolic acidosis:  Anion gap = Na+ - (Cl- + HCO-3) = 8-12 mEq/L

Elevated

Normal

Decreased

 

 

-sodium concentration falls while the chloride plus bicarbonate concentrations are unchanged

-serum sodium concentration remains normal while the serum chloride plus bicarbonate concentrations are increased

-methanol

-uremia

-diabetic ketoacidosis

-paraldehyde

-phenformin

-Iron

-INH

-lactic acidosis

-ethanol, ethylene glycol

-salicylates

(MUDPILES)

I. Renal Causes

       A. Bicarbonate loss

         Proximal renal tubular acidosis

         (RTA), type II (can be part of Fanconi’s Syn)

         Dilutional acidosis

         Carbonic anhydrase inhibitors

         Primary hyperparathyroidism

       B. Failure of bicarbonate regeneration

         Distal RTA, type I

         Distal RTA, type IV

         Diuretics: amiloride, spironolactones

II. Gastrointestinal Causes

       Diarrheal states

       Small bowel drainage

       Ureterosigmoidostomy

 III. Acidifying Salts

       Ammonium chloride

       Lysine hydrochloride

       Arginine hydrochloride

       Parenteral hyperalimentation

-multiple myeloma (IgG)

-hypoalbuminemia

-rarely: lithium intoxication, hypermagnesemia, and hypercalcemia

Lecture (11/29/00), Cecil’s


 

6.     Wound repair

  1. Removal of debris

-begins in early stages of inflammation

-initiated by liquefaction and removal of dead cellular material and other debris

  1. Formation of granulation tissue

-highly vascular, newly formed connective tissue consisting of capillaries and fibroblasts

-fills defects created by liquefaction of cellular debris

  1. Scarring

-collagen produced by fibroblasts progressively increases

-tissue becomes progressively less vascular and cellular

-contraction of wound occurs, can result in deformity of original structure

 

BRS Pathology; Cecil’s

 


 

8.     Menstrual disorders (e.g. amenorrhea, abnormal uterine bleeding)

 

Normal Menstration

·       Average age of menarche is 12 yrs; 9-16 is normal range

·       Cycle averages  28 days: 14 days of follicular phase, ovulation and 14 days of luteal phase

o       Normal range is 25-34 days

o       Polymenorrhea: cycle less that 21 days

o       Oligomenorrhea: cycle longer than 45 days

·       Normal menstral flow lasts 5 days; range is 2-8 days

o       Hypomenorrhea: very short and scant menses

o       Hypermenorrhea/Menorrhagia: long periold of flow or heavy amount of fluid

o       40 ml average flow. > 80 ml abnormal. Ask about # and frequency of pad/tampon use instead

·       Symptoms associated w/ normal menstration

o       Mittleschmertz (middle pain): small pain sometimes felt during ovulation. May be from abdominal irritation from fluid release @ ovulation.

o       Molimina: the variety of symptoms experienced before menstration. Includes weight gain, breast tenderness, edema, and irritability. Pre-Menstral Syndrome (PMS) if exaggeraged or troublesome.

o       Dysmenorrhea: menstral cramping. Usually mild, well treated w/ NSAIDs. In some women, cramping is excessive and limits activity.

Causes of Abnormal Uterine Bleeding

Compli-cations of pregnancy

-one of most common causes of abnormal bleeding

-1st trimester bleeding causes: ectopic pregnancy, miscarriage (see below), trophoblastic disease

Threatened abortion: uterine bleeding, but cervix remains closed

Inevitable abortion: uterine bleeding and dilated cervix

Incomplete abortion: uterine bleeding where some by not all tissue passed. Often requires surgical intervention (D&C)

Complete abortion: uterine bleeding with passage of all uterine contents

(Missed abortion: no viable pregnancy, seen on US, but no abnormal S/Sx)

Organic lesions

Leiomyomas

-distort and exaggerate surface area of the endometrium

-cause heavier bleeding at usual time of menses

Cervical carcinoma

-irregular, unpredictable bleeding

Vulvular/vaginal carcinoma

-irregular, unpredictable bleeding

Endometrial/ endocervical polyps

-benign overgrowths of endometrium or cervical epithelium

-spotting (inter-menstral bleeding), post-coital bleeding

Pelvic infection

-all types of infections can cause (cervicitis, endometritis, PID)

-usually between menstral cycles

Hormonal disorders

Disruption in HPO Axis can cause hormonal imbalance

-causes intermittently shedding endometrium

-normal physiology: ovulation is critical to normal pattern of bleeding. Estrogen in follicular phase lead to endometrial proliferation. Progesterone in luteal phase (and decreased estrogen) allows normal endometrial shedding

-estrogen w/o  progesterone -> constant state of proliferating endometrium, starts to partially and intermittently shed, causing abnormal bleeding

Anovulaotry states or intermittently ovulatory states

-seen near extremes of menarche and menopause (beginning and end)

-lack of ovulation causes irregularly paced periods and irregular patterns of endo sloughing

Exogenous estrogen use w/o progesterone

-usually seen with estrogen replacement or oral contraceptives

-balance exogenous estrogen w/ exogenous progesterone to provide normal bleeding pattern

Constitu-tional disorders

Bleeding disorders

-heavy menses can be presenting complaint of young women w/ von Willebrand’s Disease

Hypothyroidism

-can lead to anovulation and abnormal bleeding

Hyperprolactinemia

-can cause amenorrhea

-can be caused by prolactinoma, breast feeding, or certain meds (e.g. antipsychotics)

Liver disease

-severe disease: hormones not degraded properly -> imbalanced hormone levels and abnormal bleeding patterns

DIAGNOSIS AND MANAGEMENT OF ABNORMAL UTERINE BLEEDING

General

H&P: very important in all patients

Labs: Pap smear, CBC, pregnancy test, endometrial biopsy, hormonal testing (estrogen, progesterone, FSH, LH, prolactin, etc)

Adolescent

Anovulation: often due to immaturity of hypothalamic pituitary axis

-tx: birth control pills to exogenously impose regular cycles, self-regulation occurs in time

Pregnancy: ask (confidentially) if they are sexually active

Pelvic exam: on kids and non-sexually active can be traumatic. Use ultrasound instead.

Blood dyscrasias: can be cause of primary heavy bleeding problems

Management for most adolescent disorders is hormonal to regulate cycle

Endometrial sampling is usually not indicated for this age (hyperplasia/neoplasia rare)

Childbearing/ Reproductive age

Pregnancy complications and organic lesions: check TSH and prolactin (Sx can be subtle)

Endometrial biopsy or D&C: indicated for age 30-35 due to ­ risk of hyperplasia/malignancy

Management is primarily hormonal manipulation (combo or progesterone pill)

NSAIDs for menorrhagia for pain and reducing bleeding (¯ prostaglandin ® ¯ bleeding)

Surgical intervention: hysterectomy or endometrial ablations to control bleeding; also removal of fibroids

Peri-menopausal

Rule out malignant/pre-malignant conditions of cervix and uterus: risk ­ with age

-Pap smears and endometrial biopsy become important

Hormonal management ONLY if pt has non-malignant condition

-Oral contraceptives NOT used for women smokers > 35 (risk of clotting problems)

Surgical tx indicated if medical tx fails

-D&C: primarily diagnostic, also therapeutic for some endometrial polyps

-Hysterectomy: therapeutic for many bleeding etiologies

-Endomentrial ablation: to remove endometrium and control bleeding

Transition from normal cycling to menopause: frequency often decrease, can increase - need to rule out if just transition or pathology (e.g. cancer)

 

Causes of Amenorrhea

Primary amenorrhea

-Definition: lack of menses by age 16

-takes ~ 2 yrs from start of growth of pubic hair to start of menses

Secondary amenorrhea

-Definition: cessation of menses for a period of time (usually 3 cycles)

-pregnancy or hormonal imbalance etiologies most likely

-causes: disruption of HPaxis, anorexia, excessive exercise, some drugs, stress

Differential diagnosis

Physiologic: pregnancy, breastfeeding, menopause

Anatomic: anatomic dysfunctions on any level of system

-Uterovaginal anomalies, ovarian anomalies

-pituitary dysfuction - due to tumors, irradiation, surgery or Sheehan’s syndrome (infarction)

-hypothalamic or CNS

-Hypothyroidism: ­ TSH also stimulates prolactin release

Amenorrhea Diagnosis and Treatment

Diagnosis

-Primary amenorrhea: where is pt in rest of sexual development (breast buds, axillary/pubic hair?)

-Excessive exercise: can become hypoestogenic  ® reduced bone density

-Embryology: lower 2/3 of vagina formed from urogenital sinus, upper 1/3 from fused mulerian ducts. If structures don’t form correctly or fuse properly, pt can have imperforate hymen or vaginal atresia.

Imperforate hymen: pts menstrate but don’t experience external bleeding

-experience cyclic symptoms of menstration, but present w/ 1° amenorrhea

-PE: imperforate hymen that is usually bulging outward w/ blood

-Tx: correct surgically by making X-shaped incision, will menstrate normally

-higher risk for endometriosis due to retrograde menstral flow

Congenital absence of uterus and vagina: failure of mulerian ducts to form properly.

-have functional ovaries and normal hormonal function

-Associated renal and pelvic abnormalities

-blind vaginal dimple (remnant from urogenital sinus)

-create vagina by stretching pouch w/ direct pressure using dilator over several months

-once sexually active, dilator no longer needed (use periodically if not)

Androgen insensitivity: genotypically male, phenotypically female; lack of testosterone receptor

-have normal breast development, sparse axillary/pubic hair and a vaginal dimple

-have testes, not ovaries – need to be removed surgically due to risk of malignancy

-aka testicular feminization or male pseudohermaphroditism         (think Jamie Lee Curtis)

Turner’s Syndrome: 45 XO or mosaic genotype

-characteristic webbed neck, shield chest, cubitus valgus, sexual infantilism

-do not have functioning ovaries, do no menstrate properly

Polycystic Ovarian Syndrome: obesity, hirsutism, and acne are classic triad w/ amenorrhea

-tend to be hyperandrogenic, exact etiology unknown

-anovulation and oligomenorrhea or amenorrhea

-begin to put on weight in teens or early 20’s

-losing weight helps condition, but difficult for pts to do (insulin resistance contributes?)

Work-up

Ultrasound: used to examine ovaries

Labs:Serum prolactin and TSH, pregnancy test, karyotyping, androgen levels, FSH/LH

MRI: used to image pituitary to look for tumors (e.g. prolactinoma)

Progesterone challenge: adminster progesterone for 5 days, followed by withdrawal of progesterone. Induces menses if uterus and outflow tracts are normal

Endometrial sampling: using biopsy or D&C to check if lining of uterus is appropriately proliferative or secretory, presence of hyperplasia/carcinoma

Treatment

-dependent on cause

Hormonal imbalance: cyclic progestational agents to mimic luteal phase and regulate menstration or complete exogenous regulation w/ combo OCPs

Goals: correct hypoestrogenism, prevent endometrial hyperplasia, correct elevated prolactin levels

-can include surgical “reconstruction”, hormonal manipulation, nutrition/exercise counseling

 

Lecture (4/16/01)


 

9.     Cell injury and death

 

·       Ischemic cell injury

o      Causes: Obstruction of arterial blood flow, Anemia, CO poisoning, decreased perfusion, poor oxygenation of blood

o      Early stages – mitochondria affected, decreased oxidative phosphorylation and ATP synthesis

§       Failure of cell membrane pump – hydropic change, swelling of ER, swelling of mitochondria

§       Disaggregation of ribosomes and failure of protein synthesis

§       Stimulation of phosphofructokinase activity – increased glycolysis, lactate, acidification

o      Late stages – membrane damage, reversible

§       Myelin figures – whorl-like, from damaged membranes

§       Cell blebs – from disorderly fxn of cytoskeleton

o      Cell death

§       Irreversible damage to mito and cell membranes – massive Ca++ influx

§       AST, CPK, and LDH into blood are indicators of injury to heart muscle

§       Vulnerability varies w/ tissue type: neurons (3-5 min), myocardial cells and hepatocytes (1-2 hrs), skeletal muscle (many hrs)

·       Free radical injury

o      Generation by normal metabolism, oxygen toxicity (e.g. ARDS, retrolental fibroplasias), ionizing radiation, drugs and chemicals, reperfusion after ischemic injury

o      Free radical degradation occurs by intracellular enzymes (glutathione peroxidase, catalase, superoxide dismutase), endogenous substances (ceruloplasmin, transferring), spontaneous decay

·       Chemical cell injury (e.g. liver cell membrane damage by CCl4)

o      Processed by P-450s to make highly reactive CCl3·

o      CCl3· initates lipid peroxidation of intracellular membranes

§       Disaggregation of ribosomes -> decreased protein synthesis, accumulation of intracellular lipids (fatty change)

§       Plasma membrane damage -> cellular swelling, massive influx of Ca++, mitochondrial damage

·       Necrosis

o      Coagulation necrosis

§       Sudden cutoff of blood supply to organ (esp. heart, kidney)

§       Preservation of tissue architecture in early stages

§       Increased cytoplasmic eosinophilia due to protein denaturation

§       Marked nuclear changes

·       Pyknosis – chromatin clumping, shrinking w/ increased basophilia

·       Karyorrhexis – fragmentation

·       Karyolysis - fading

·       Disappearance of stainable nuclei

o      Liquefaction necrosis

§       Softening of tissue

§       From ischemic injury to central nervous system by autolysis, suppurative infections

o      Caseous necrosis

§       Combine coagulation and liquefaction necrosis

§       Cheese-like on gross exam

§       Amorphous eosinophilic appearance

§       Part of granulomatous inflammation (e.g. TB)

o      Gangrenous necrosis

§       Most often lower extremities or bowel

§       Secondary to vascular occulusion

§       “Wet” when complicated by infective heterolysis and liquefaction necrosis

§       “Dry” when mostly coagulation necrosis w/o liquefaction

o      Fibrinoid necrosis

§       Immune-mediated vascular damage

§       Deposition of fibrin-like proteinaceous material in arterial walls

o      Fat necrosis

§       Traumatic: following severe injury to tissue w/ high fat content (e.g. breast)

§       Enzymatic: complication of acute hemorrhagic pancreatitis

·       Pancreatic enzymes diffuse into tissue, digest parenchyma

·       Liberated fatty acids form calcium salts (saponification)

·       Vessels eroded -> hemorrhage

o      Apoptosis – programmed cell death

§       Shrinkage and increased acidophilic staining

§       Physiologic process for removal of cells during embryogenesis, programmed cell cycling

 

BRS Pathology


 

10.  Malabsorption (e.g. celiac sprue, bacterial overgrowth, disaccharide deficiency)

 

Disease

morphology

other

Celiac sprue

-Flat mucosal surface w/ villous atrophy

-increased lymphocytes and plasma cells in lamina propria

-Immune-mediated gluten sensitivity

-associated w/ HLA-B8 and HLA-DW3

-can lead to B-cell lymphoma in small intestine

-weight loss, weakness, diarrhea

-pale, bulky, frothy foul-smelling stool

Tropical sprue

-histological finding vary (can be like celiac)

-probable infectious origin

-often responds to antibiotics

Whipple’s disease

-PAS-positive macrophages in intestinal mucosa

-bacteria-like inclusions by EM

-may affect any organ

Disaccharidase deficiency

-no histological changes

-deficiency in brush border disaccharideases

-Lactase deficiency: milk intolerance, most frequent

-flatus, osmotic diarrhea

Bacterial overgrowth

-colon-like growths and populations of bacteria in small bowel

-occurs in areas of stasis (causes: surgery, fistula, diverticuli, strictures, etc)

abetalipoproteinemia

-circulating acanthocytes suggest diagnosis

-hereditary deficiency of apoprotein B

Intestinal lymphangiectasia

-generalized dilation of small intestinal lymphatics

-gastrointestinal protein loss

-hypoproteinemia, generalized edema

Chronic pancreatitis

-destruction and fibrosis of pancreas

-impaired secretion of pancreatic enzymes, fluid, HCO3

-often due to long-standing alcohol use

-weight loss, poor appetite, oily bulky stools, edema, hypoproteinemia

BRS Pathology; lecture (1/10/01)