3. Micro Integrated Flow Cytometer (MIFC)

Yi-Chung Tung

This project develops a miniaturized integrated flow cytometer that incorporates a micro-channel flow cell and a self-aligned micro optical system made of a disposable elastomer. This MEMS-based flow cytometer device is named the “Micro Integrated Flow Cytometer (MIFC).” The objectives of this research are to explore new microsystem/MEMS designs and microfabrication processes needed for the development of the MIFC and to provide technological solutions to the challenges associated with the integration of the MIFC components. This research also proposes a new MEMS self-integrated optical system, including micro lens and micro prisms, which allow simultaneous multi-color detection with high resolution in the visible wavelength spectrum. Furthermore, a new MEMS structure that will allow multi-channel sample detection using a micro-actuated optical excitation device is also proposed in this research.

Figure 2. Hydrodynamic focusing of fluorescent particles in the microchannel flow cell of the MIFC.

Collaboration

Group of Professor Skerlos, Mechanical Engineering Department, University of Michigan

Group of Professor Takayama, Biomedical Engineering Department, University of Michigan

Recent Publications

1. Huh, D., Tung, Y-C., Grotberg, J.B., Skerlos, S., Kurabayashi, K., and Takayama, S., “Air-Liquid Two-Phase Microfluidic System for Low-Cost, Low-Volume, and Low-Power Micro Flow Cytometer,” Proc. the 5th International Symposium on Micro Total Analysis Systems (µTAS), Monterey, CA, October 21-25, 2001.

2. Tung, Y.-C., Lin, C.-T., Kurabayashi, K., Skerlos, S.J., “High Fidelity and Low Cost Detection of Mulit-Color Fluorescence from Biological Cells in a Micro Integrated Flow Cytometer (MIFC) with Disposable Observation Cell,” Proc. the 6^th International Symposium on Micro Total Analysis Systems (mTAS), Nara, Japan, November, 3-7, 2002.

Sponsorship

University of Michigan College of Engineering, ISET Program

NSF CAREER