Coherent Raman Microscopy

Pulse-shaping Fluorescence Microscopy

Since it first revealed the existence of cells, the microscope has remained one of our most important tools for learning about the biological world. Early microscopes exploited density differences to produce images, but these images contained no chemical information. An endogenous form of contrast that exists for every molecule is its Raman spectrum. Raman spectra, with well-defined transitions in the "fingerprint" region can readily identify different chemical species, providing an exciting mode of contrast for molecular imaging... read more here

Broadband femtosecond laser sources have been frequently used in multiphoton fluorescence microscopy. An ultrabroadband source (>100nm) can access many fluorophores, but lacks the ability to do so selectively. Pulse-shaping techniques employed in the coherent control community can solve this problem...

read more here