Ginger
M. Kubish
September 19, 2005
Dear Sir or Madam:
Briefly, I offer:
þ Graduate coursework in Pharmaceutical Engineering.
þ B.S. in Microbiology from Michigan State University.
þ 8 years experience as a Research Associate chiefly working as an Enzymologist/Protein Chemist.
þ 8 years experience managing well-known laboratories in Biological Chemistry at The University of Michigan.
þ Extensive experience growing bacterial and maintaining mammalian cell cultures.
þ Proven experience with aseptic technique.
þ Extensive experience with protein purification and electrophoresis.
þ Experience subcloning and modifying proteins of interest.
þ Extensive experience isolating plasmids and bacterial transformation.
þ Experience with pDNA purification, subcloning and restriction analysis.
þ Experience with Polymerase Chain Reactions (PCR).
þ Experience analyzing data from enzyme kinetic studies.
þ Experience with in vitro assays related to drug metabolism.
þ Experience using cutting-edge microarray technology for HTS.
þ Experience with immunohistochemistry.
þ Experience using fluorescence microscopy.
þ Computer Expertise.
þ Formal training- Foundations of Supervision.
þ Experience with HPLCs (Hewlett Packard Series 1050 and Waters 600 plus 996 Photodiode Array), GC, FPLC, and various spectrophotometers.
þ Active involvement with group discussions including presentations, reviews, and reports on current literature.
My compensation requirements are negotiable. I would also like to add that I am an assertive self-starter and have the ability to teach and help others. I pay great attention to detail and strive for perfection. I look forward to speaking with you at your earliest convenience. I can be reached by email at dziuragi@umich.edu.
Sincerely,
Ginger Kubish
enc. resume
Ginger
M. Kubish
Objective: I am seeking a position in
Industry to further enhance my Enzymology skills.
Related Work
Experience
Research
Associate I, University of Michigan -Ann Arbor, MI
Dr.
Michael D. Uhler Laboratory (05/01-present)
· Optimization of Surface Transfection and Expression Protocol (STEP).
· Identification of the inhibitory domain of cGMP-dependent protein kinase using STEP to screen a mutational expression library.
· STEP optimization for cellular delivery of antisense oligonucleotides.
· Development of a penton-histone protein.
· Laboratory Manager (including MSDS and radiation manager, safety coordinator, and purchaser)
· Supervisor to 9 workstudy students; Provider of training for rotating graduate students.
Skills Necessary for this Position
·
Strong Communication and Organizational skills.
· Biotechnology: Microarray technology and instrumentation (Genomic Solutions, BioRobotics), liquid handling systems, and digital imaging and detection.
·
Molecular Biology:
CsCl based plasmid purification, transfection via electroporation, PCR,
primer construction, DNA/RNA clean-up, XL1-Blue competent cell preps,
immunoassays, pDsRed and pEGFP expression vectors, and electrophoresis.
·
Biochemistry: instrumentation (UV-visible spectroscopy,
Beckman scintillation counter).
·
Microbiology:
Bacterial (E. coli) and mammalian cell (HEK 293T, PKCaEGFP,
Jurkat, S49, N2A, A7R5, PC12 and P19) cultures.
·
Computer Skills: Windows NT and XP, MacOS,
lasergene/dnastar, MS Outlook, Word Processing (MS Word, Notepad), Graphics
software (MS Power Point, MS Excel), Imaging software (MCID, AIS,
IntelliQuant), and Internet software (Netscape, Internet Explorer).
Duties/Responsibilities
· Purification of Cytochrome P450s and Cytochrome P450 reductase.
· Assisted with RES assays.
· Data and statistical analysis for the entire laboratory; Preparation of materials for publication.
· Laboratory Manager (MSDS manager, safety coordinator, and Purchaser)
Skills Necessary for this
Position
· Strong Communication and Organizational skills.
· Molecular Biology: plasmid purification, transformation, restriction enzyme digestion, ligation, competent cell prep, conjugation, overexpression plasmid construction, and DNA sequencing.
· Biochemistry: instrumentation (UV-visible spectroscopy), protein purification (affinity, ion exchange, hydroxyapatite, and size exclusion chromatography), protein assay, SDS-PAGE, and Western Blot.
· Microbiology: Bacterial cultures.
· Computer
Skills: Windows 95, 97 and 2000, MacOS, word processing (MS Word, Endnote,
Reference Manager), Graphics software (MS Power Point, MS Excel, MacDraw,
ChemWindow, Smartdraw, Prism, Corel Draw), Internet software (Netscape,
Internet Explorer) and HTML.
Research
Assistant, Whatman.-Ann Arbor, MI (2/96-12/96)
Duties/Responsibilities
· Developed a leukocyte reduction filter
· Performed blood filtration experiments
· Collected and interpreted data from filtration experiments
· Data and statistical analysis
· Maintained red blood cell samples
· Prepared graphs for presentation
Skills Necessary for this
Position
· Reagent preparation Serial dilution
· Database management
· Expertise in Microsoft Excel and Word
· Microscopic analysis
Laboratory
Technician, MSU Department of Microbiology -E. Lansing, MI (5/92-11/95)
Duties/Responsibilities
· Purified urease, via column chromatography.
· Prepared media and stock solutions for laboratory personnel.
· Prepared various protein and DNA gels.
· Washed
and sterilized laboratory glassware.
Department
of Microbiology (4/95-9/95) and (8/93-12/93)
· Topic: Studies of the enzymatic mechanisms of urease to understand why closely related ureases have vastly different specific activities.
· Topic: The incorporation of 2,4 Dichlorophenoxyacetic acid by Rhizobium leguminosarum biovar trifolii strains ANU 843 and ANU 845.
Education
· Graduate coursework in Pharmaceutical Engineering at the University of Michigan.
· Bachelor
of Science in Microbiology from Michigan State University; awarded June, 1995
Publication
Microarray
transfection analysis of transcriptional regulation by cAMP-dependent protein
kinase in Molecular and Cellular
Proteomics. Molecular and
Cellular Proteomics. Aug. 2004
References
Available upon request