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NASA (JSC) Collaborations Robert G. Dennis, Ph.D. |
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In a collaboration with
Tom
Goodwin at NASA Johnson Space Center, we have developed 2-dimensional
and 3-dimensional bioreactor systems to subject tissues to controlled electromagnetic
fields. Experiments are currently in progress, and our preliminary
data is extremely promising. We have subjected normal human neural
progenitor (NHNP) cells to low level electromagnetic fields generated near
electrically conductive plate electrodes (2-D) or within a field generated
by a solenoid coil (3-D). The waveforms tested included sine waves,
narrow pulses (delta function), and square waves. Cells were subjected
to 17 days of electromagnetic field stimulation of all waveforms as well
as a DC field.
The cells were not
subjected to transverse electrical fields through the culture medium, as
is often done in cell culture experiments employing electromagnetic fields,
rather the cells were grown within the induced magnetic field surrounding
the electrical conductor. For both the 2-D and 3-D systems, the magnetic
field intensity was limited to ~ 70 mG. Magnetic field intensity
was measured using a linear Hall effect sensor. For comparison, the
Earth's magnetic field is approximately 500 mG at 45o latitude.
Rate of change of the magnetic fields was estimated on the basis of Maxwell's
equations and the measured current transient response in both the conductive
plate and the solenoid coil. The mB
(micromagnetic) fields were applied in 5 separate interventions as:
(1) 10 Hz bipolar square waves,
(2) differentiated square waves (“delta
function”); narrow pulses (200 ms)
corresponding to each square wave edge,
(3) sine waves of the same amplitude and
frequency as the square waves,
(4) DC (steady) mB
fields, also of the same amplitude as the square wave, and
(5) control (no mB
field).
Cell Responses: (relative to control)
Proliferation rate increased up to 4x
Morphology changes were macroscopically
evident for large colonies of nerve cells in 2-D
Glucose metabolism +~60% in 3-D system
Gene array profiling indicated very significant
increases in expression of classes of genes related to extra-cellular matrix
production, growth, and metabolism.
All effects were greatest for square and delta functions, no difference between DC fields and control (no field).
Conclusions: Cells respond to the rate of change in the mB field (dB/dt), not to the peak field magnitude (Bmax) or total flux exposure. The high dB/dt of the square waves and the delta were both effective at influencing cellular response, whereas slowly varying (sine) or non varying (DC) fields had significantly reduced or no effect. Equivalently high peak fields or long exposure times (sine and DC, as well as square wave) were clearly not as important as the rate of change of the mB field. In this study, peak magnetic field amplitudes were ~ 70 mG, whereas the Earth's magnetic field on average is ~ 500 mG, but is not time varying (i.e., it is DC). The electromagnetic interventions carried out in this study were of course superimposed upon the Earth's DC magnetic field. It is the time varying nature of the fields that apparently has the most significant influence on every aspect of the cellular response. We collectively term these time varying electromagnetic fields as TVEMFs.
The instrumentation and protocols for this series of experiments have been filed with the United States Patent and Trademark Office, and two manuscripts are currently in preparation.
Bob's Home Page Current Research Muscle Mechanics Lab (U of M) Biomechatronics Group @ MIT